Wednesday, October 2, 2013

Bioinformatics based databases and tools for RNA analysis:






a.  Gene Expression analysis:


Gene Expression Omnibus (GEO) is a central repository at NCBI that catalogues expression profile information from a variety of organism( http://www.ncbi.nlm.nih.gov/geo/ ). A similar database at EMBL is maintained as Arrayexpress(http://www.ebi.ac.uk/arrayexpress/). Specialized databases that are specific to organisms include eFP browser (http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi) and AtGenExpress(http://www.weigelworld.org/resources/microarray/AtGenExpress/) for Arabidopsis thalianaModmine for Drosphila melanogaster(http://intermine.modencode.org/query/portal.do?class=Project&externalids=Celniker) is maintained under the Berkley Drosophila genome project (http://www.fruitfly.org). Brain atlas is a database for expression datasets in brain tissue, irrespective of the organism.






  • A large number of RNA molecules undergo 3-D structural conformation to obtain functionality. Bioinformatic tools and databases have been created that are useful for prediction of RNA structures.RNA structure prediction:



One software that is commonly employed for RNA structure prediction is mFold (http://mfold.rit.albany.edu/?q=mfold), which accepts nucleotide sequences and predicts the all the probable structures that can be derived from the sequence. Some other RNA fold bioinformatics tools include RNAFold webserver (http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi).



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